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pbluescript ii ks (+/−) phagemid cloning vector  (Agilent technologies)


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    Structured Review

    Agilent technologies pbluescript ii ks (+/−) phagemid cloning vector
    Pbluescript Ii Ks (+/−) Phagemid Cloning Vector, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbluescript+ii+ks(%E2%88%92)+phagemid+vector/pmc00355910-66-25-33
    Average 90 stars, based on 1 article reviews
    pbluescript ii ks (+/−) phagemid cloning vector - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    other:

    Article Title: Discovery of stromal regulatory networks that suppress Ras-sensitized epithelial cell proliferation
    Article Snippet: The PCR products were digested with restriction enzymes and sequentially inserted into the pBluescript II KS(+) phagemid vector (Agilent Technologies; 212207).

    Article Title: L1 retrotransposon-mediated stable gene silencing
    Article Snippet: The neo -cassette tagged L1 RP was inserted into pBluescript II KS(−) phagemid vector (Stratagene, La Jolla, CA) to create pBSKS-L1 RP - neo .

    Article Title: Purification and Partial Characterization of an Entomopoxvirus (DlEPV) from a Parasitic Wasp of Tephritid Fruit Flies.
    Article Snippet: About 10 μg of DlEPV DNA were digested with the EcoRI (RI) restriction enzyme (Roche Molecular Biochemicals) and cloned into the EcoRI site of the pBluescript II KS (+/−) phagemid cloning vector (pBS; Stratagene, www.stratagene.com/ ) using standard protocols ( ).

    Article Title: Monoglyceride Lipase Deficiency in Mice Impairs Lipolysis and Attenuates Diet-induced Insulin Resistance
    Article Snippet: To generate the MGL gene targeting vector, a synthetic oligonucleotide encoding a lox P sequence flanked by the EcoRV restriction site at the 5′ end and KpnI and HindIII restriction site at the 3′ end, respectively, was digested with EcoRV and HindIII restriction enzymes and ligated into pBluescript II KS(−) phagemid vector (Stratagene, La Jolla, CA).

    Article Title: Genetic Interaction of OsMADS3DROOPING LEAFand OsMADS13 in Specifying Rice Floral Organ Identities and Meristem Determinacy
    Article Snippet: For the construction of specific probes for OsMADS13 , SPW1 / OsMADS16 , and DL , gene-specific fragments of OsMADS13 cDNA (367–958 bp), OsMADS16 cDNA (211–686 bp), and DL cDNA (121–639 bp) were amplified by reverse transcription (RT)-PCR using primers 13PPF/13PPR, 16PPF/16PPR, and DLPPF/DLPPR, respectively ( Supplemental Table S1 ) and cloned into pBluescript II KS+ phagemid vector (Stratagene).

    Article Title: A Novel Role of RASSF9 in Maintaining Epidermal Homeostasis
    Article Snippet: Fragments corresponding to nucleotides (nt) 285–817 and 1126–1625 of the mouse RASSF9 cDNA (NCBI RefSeq NM_146240) were separately cloned into the Eco RI- Xho I sites of the pBluescript II KS (+) phagemid vector (Stratagene, La Jolla, CA) for riboprobe synthesis.

    Article Title: Antibodies Directed toward Neuraminidase N1 Control Disease in a Mouse Model of Influenza
    Article Snippet: PCR products were purified using the Isolate II PCR and gel kit (Bioline) and cloned in the pBluescript II Phagemid KS+ vector (Agilent Technologies) by blunt-end ligation using T4 ligase (Fermentas).

    Clone Assay:

    Article Title: Haplotype Analysis and Linkage Disequilibrium at Five Loci in Eragrostis tef
    Article Snippet: .. Clones within the contig that hybridized to the sd1 or rht1 probes and amplified with the sd1 or rht1 primers were digested with Not I, gel purified (QIAGEN) and subcloned into the pBluescript II KS phagemid vector as described by the manufacturer (Stratagene). ..

    Amplification:

    Article Title: Haplotype Analysis and Linkage Disequilibrium at Five Loci in Eragrostis tef
    Article Snippet: .. Clones within the contig that hybridized to the sd1 or rht1 probes and amplified with the sd1 or rht1 primers were digested with Not I, gel purified (QIAGEN) and subcloned into the pBluescript II KS phagemid vector as described by the manufacturer (Stratagene). ..

    Purification:

    Article Title: Haplotype Analysis and Linkage Disequilibrium at Five Loci in Eragrostis tef
    Article Snippet: .. Clones within the contig that hybridized to the sd1 or rht1 probes and amplified with the sd1 or rht1 primers were digested with Not I, gel purified (QIAGEN) and subcloned into the pBluescript II KS phagemid vector as described by the manufacturer (Stratagene). ..



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